Journal: PLoS ONE

Article Title: IL-6 Induced STAT3 Signalling Is Associated with the Proliferation of Human Muscle Satellite Cells Following Acute Muscle Damage

doi: 10.1371/journal.pone.0017392

Figure Lengend Snippet: ( 6a ) cMyc mRNA relative to GAPDH, expression represented as fold changes from PRE. ( 6b ) cMyc + SC as a percentage of Pax7 + cells between Pre and T24, n = 7. ( 6c & 6d ) Serial sections of the same muscle cross-section with higher magnification (100×) boxes showing the same nuclei in both images. ( 6c ) Representative image at 20× magnification of a merged Pax7/Laminin costain where ( c-i ) Pax7 is in green, ( c-ii ) DAPI + nuclei are in blue and ( c-iii ) laminin in red. Note the two Pax7 + nuclei which have been highlighted to show their individual Pax7 positivity, DAPI staining and location within the laminin boarder. These nuclei are highlighted again in ( 6d ) where the same cells have been stained colourometrically for cMyc. (d-i) A magnified image of the inset box showing the brown cMyc staining co-localized to the nuclear marker hematoxalin The brown cMyc positive cells are clearly within the visible fiber boarder. *p<0.05 vs. PRE.

Article Snippet: 7 μm muscle cross-sections were stained with antibodies against Pax7 (neat; cell supernatant from cells obtained from the DSHB, Iowa City USA); IL-6 (500 ng/mL, MAB 2061, R&D Systems, Minneapolis USA); p-STAT3 (1∶100, Cell Signaling Technologies Inc., Danvers USA) and laminin (1∶1000, L8271, Sigma-Aldrich, Oakville Canada).

Techniques: Expressing, Staining, Marker

Journal: Experimental and Therapeutic Medicine

Article Title: Calcimimetic R568 reduced the blood pressure and improved aortic remodeling in spontaneously hypertensive rats by inhibiting local renin-angiotensin system activity

doi: 10.3892/etm.2018.6734

Figure Lengend Snippet: Immunohistochemical analysis of proliferating remodeling protein expression in the thoracic aorta of rats. Representative images of (A) Calponin; (B) α-SMA, smooth muscle α-actin; (C) OPN, osteopontin; (D) PCNA, proliferating cell nuclear antigen. Quantification of (E) Calponin; (F) α-SMA, smooth muscle α-actin; (G) OPN, osteopontin; (H) PCNA, proliferating cell nuclear antigen. Data are means ± standard deviation (n=7). SHR+NS groups vs. the age-matched WKY+NS groups, *P<0.05; SHR16w+NS groups vs. SHR8w+NS groups, # P<0.05; SHR+R568 groups vs. the age-matched WKY+R568 groups, **P<0.05; SHR16w+R568 groups vs. SHR8w+R568 groups, ## P<0.05; SHR+R568 groups vs. SHR+NS groups, a P<0.05. WKY, Wistar Kyoto rats; SHR, spontaneously hypertensive rats; NS, normal saline; R568, NPSR568; α-SMA, smooth muscle α-actin; OPN, osteopontin; PCNA, proliferating cell nuclear antigen. Scale bar, 50 µM.

Article Snippet: The sections were incubated with primary mouse polyclonal antibodies against CaSR (1:50; Abcam, MA, USA), calponin (1:600; Boster, Wuhan, China), smooth muscle actin α (α-SMA, 1:400; Boster), proliferating cell nuclear antigen (PCNA, 1:200; Boster), and primary rabbit monoclonal antibody against osteopontin (OPN, 1:100; Abcam) overnight at 4°C.

Techniques: Immunohistochemical staining, Expressing, Standard Deviation, Saline

Journal: Experimental and Therapeutic Medicine

Article Title: Calcimimetic R568 reduced the blood pressure and improved aortic remodeling in spontaneously hypertensive rats by inhibiting local renin-angiotensin system activity

doi: 10.3892/etm.2018.6734

Figure Lengend Snippet: Western blot analysis of proliferating remodeling protein expression in the thoracic aorta of rats. (A) Western blot analysis of the protein expression. (B) Calponin. (C) α-SMA, smooth muscle α-actin. (D) OPN, osteopontin. (E) PCNA, proliferating cell nuclear antigen. Data are means ± standard deviation (n = 7). SHR+NS groups vs. the age-matched WKY+NS groups, *P<0.05; SHR16w+NS groups vs. SHR8w+NS groups, # P<0.05; SHR+R568 groups vs. the age-matched WKY+R568 groups, **P<0.05; SHR16w+R568 groups vs. SHR8w+R568 groups, ## P<0.05; SHR+R568 groups vs. SHR+NS groups, a P<0.05. WKY, Wistar Kyoto rats; SHR, spontaneously hypertensive rats; NS, normal saline; R568, NPSR568; α-SMA, smooth muscle α-actin; OPN, osteopontin; PCNA, proliferating cell nuclear antigen.

Article Snippet: The sections were incubated with primary mouse polyclonal antibodies against CaSR (1:50; Abcam, MA, USA), calponin (1:600; Boster, Wuhan, China), smooth muscle actin α (α-SMA, 1:400; Boster), proliferating cell nuclear antigen (PCNA, 1:200; Boster), and primary rabbit monoclonal antibody against osteopontin (OPN, 1:100; Abcam) overnight at 4°C.

Techniques: Western Blot, Expressing, Standard Deviation, Saline